LOCUS       sequence-110220-        1768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes 6XHis_3XFlag as a Type 4a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK060
FEATURES             Location/Qualifiers
     source          1..1768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(133..167)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(238..897)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     812..831
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1079..1667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1568..1587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccgattaca aggacgatga tgataaggac tacaaagatg atgatgacaa
       61 agattataag gacgatgatg ataaaggtca tcatcatcat catcactaac tcgagtggct
      121 gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      181 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      241 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      301 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      361 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      421 gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc
      481 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      541 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      601 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      661 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      721 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      781 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      841 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      901 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
      961 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt
     1021 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1081 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1141 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1201 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1261 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1321 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1381 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1441 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1501 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1561 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1621 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1681 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     1741 tatcccctga ttctgtggat aaccgtag
//
