LOCUS       sequence-110223-        1891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes tADH1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK063
FEATURES             Location/Qualifiers
     source          1..1891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      14..201
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(69..93)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(256..290)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(361..1020)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     935..954
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1202..1790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1691..1710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat ggcgcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
       61 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
      121 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
      181 tattgaccac acctctaccg gcatgccgag caaatgcctg caaatcgctc cccatttcgc
      241 tgtgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      301 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
      361 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      421 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      481 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      541 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt
      601 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      661 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      721 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      781 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      841 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      901 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      961 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1021 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1081 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc
     1141 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1201 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1261 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1321 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1381 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1441 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1501 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1561 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1621 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1681 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1741 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1801 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1861 cgttatcccc tgattctgtg gataaccgta g
//
