LOCUS       sequence-110234-        2725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes URA3 as a Type 6 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK074
FEATURES             Location/Qualifiers
     source          1..2725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             191..994
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      complement(1090..1124)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1195..1854)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1769..1788
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2036..2624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2525..2544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat acacggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga
       61 agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga
      121 aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacgt gcaggaaacg
      181 aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt
      241 tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga
      301 tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt
      361 actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct
      421 aaaggcatta tccgccaagt acaatttttt actcttcgag gacagaaaat ttgctgacat
      481 tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga
      541 cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc
      601 agaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg
      661 ctccctatct actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga
      721 ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg
      781 gttgattatg acacccggtg tgggtttaga tgacaaggga gatgcattgg gtcaacagta
      841 tagaaccgtg gatgatgtgg tttctacagg atctgacatt attattgttg gaagaggact
      901 atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga
      961 agcatatttg agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg
     1021 tatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac ccgagttgag
     1081 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1141 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1201 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1261 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1321 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1381 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     1441 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1501 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1561 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1621 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1681 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1741 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1801 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     1861 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1921 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
     1981 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2041 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2101 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2161 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2221 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2281 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2341 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2401 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2461 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2521 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2581 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2641 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2701 cccctgattc tgtggataac cgtag
//
