LOCUS       sequence-110236-        2776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes HIS3 as a Type 6 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK076
FEATURES             Location/Qualifiers
     source          1..2776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        63..250
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             251..913
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     terminator      complement(1141..1175)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1246..1905)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1820..1839
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2087..2675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2576..2595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat acaaacacag tcctttcccg caattttctt tttctattac tcttggcctc
       61 ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt ttttttccac
      121 ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg aattatacat
      181 tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg caagataaac
      241 gaaggcaaag atgacagagc agaaagccct agtaaagcgt attacaaatg aaaccaagat
      301 tcagattgcg atctctttaa agggtggtcc cctagcgata gagcactcga tcttcccaga
      361 aaaagaggca gaagcagtag cagaacaggc cacacaatcg caagtgatta acgtccacac
      421 aggtataggg tttctggacc atatgataca tgctctggcc aagcattccg gctggtcgct
      481 aatcgttgag tgcattggtg acttacacat agacgaccat cacaccactg aggactgcgg
      541 gattgctctc ggtcaagctt ttaaagaggc cctaggggcc gtgcgtggag taaaaaggtt
      601 tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggttg atctttcgaa
      661 caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggtg atctctcttg
      721 cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta ccctccacgt
      781 tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca aggctcttgc
      841 ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct ccaccaaagg
      901 tgttcttatg tagtgacacc gattatttaa agctgctgca tacgatatat atacatgtgt
      961 atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat actgaagatg
     1021 acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt ccttttttct
     1081 ttttgctttt tctttttttt tctcttgaac tcgacggatc atagagttga gaccagacca
     1141 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1201 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1261 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1321 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1381 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1441 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     1501 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1561 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1621 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1681 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1741 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1801 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1861 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1921 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1981 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
     2041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2761 ctgtggataa ccgtag
//
