LOCUS       sequence-110237-        3061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes KanamycinR as a Type 6 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK077
FEATURES             Location/Qualifiers
     source          1..3061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            53..1408
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     CDS             397..1206
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(464..483)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1211..1408
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     terminator      complement(1426..1460)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1531..2190)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2105..2124
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2372..2960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2861..2880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcct acagtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
       61 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      121 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      181 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctc cagacctgcg agcagggaaa
      241 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      301 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
      361 atacagttct cacatcacat ccgaacataa acaaaaatgg gtaaggaaaa gactcacgtt
      421 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
      481 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
      541 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
      601 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
      661 cctgatgatg catggttact caccactgcg atccccggca aaacagcatt ccaggtatta
      721 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
      781 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctggct
      841 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
      901 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg
      961 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1021 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     1081 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     1141 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     1201 ttctaaagta actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
     1261 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
     1321 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
     1381 tcgtatgtga atgctggtcg ctatactgga gttgagacca gaccaataaa aaacgcccgg
     1441 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     1501 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     1561 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     1621 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1681 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     1741 ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1801 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1861 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     1921 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     1981 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     2041 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     2101 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     2161 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     2221 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     2281 tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2341 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2401 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2461 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2521 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2581 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2641 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2701 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2761 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2821 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2881 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2941 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3001 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3061 g
//
