LOCUS       sequence-110238-        2824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes NourseothricinR as a Type 6 part to be used in the Dueber 
            YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK078
FEATURES             Location/Qualifiers
     source          1..2824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      974..1171
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     terminator      complement(1189..1223)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1294..1953)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1868..1887
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2135..2723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2624..2643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcct acagtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
       61 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      121 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      181 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctc cagacctgcg agcagggaaa
      241 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      301 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
      361 atacagttct cacatcacat ccgaacataa acaaaaatgg gtactacctt agatgataca
      421 gcctacagat acagaacatc agtccctggt gatgctgaag caattgaggc tttagacggt
      481 tcattcacca ccgacaccgt ctttagagta accgccaccg gtgatggatt taccttaaga
      541 gaagtcccag tcgaccctcc attaactaaa gtctttccag atgatgaatc tgatgacgaa
      601 agcgacgacg gagaagatgg tgacccagat tcaagaactt tcgtagcata cggtgatgac
      661 ggtgatttgg ctggttttgt agtcgtttct tattcaggtt ggaatagaag gttgaccgtt
      721 gaagatatag aagtcgcccc agagcataga ggtcatggtg taggaagagc tttgatgggt
      781 ttggctacag aatttgcaag agagagagga gccggtcatt tatggttaga agttactaat
      841 gttaacgccc ctgctatcca tgcttataga agaatgggtt tcacattatg tggtttagat
      901 actgctttat atgatggaac agcatctgac ggtgaacagg ccttgtatat gtctatgcct
      961 tgcccttaaa gtaactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata
     1021 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt
     1081 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt
     1141 caatcgtatg tgaatgctgg tcgctatact ggagttgaga ccagaccaat aaaaaacgcc
     1201 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1261 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     1321 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1381 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1441 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1501 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
     1561 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1621 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1681 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1741 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1801 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1861 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1921 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1981 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     2041 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2281 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2821 gtag
//
