LOCUS       sequence-110240-        2626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ZeocinR as a Type 6 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK080
FEATURES             Location/Qualifiers
     source          1..2626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      776..973
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     terminator      complement(991..1025)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1096..1755)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1670..1689
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2426..2445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat acagtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
       61 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      121 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      181 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctc cagacctgcg agcagggaaa
      241 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      301 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
      361 atacagttct cacatcacat ccgaacataa acaaaaatgg ctaaattaac atctgccgtt
      421 cctgttttaa cagctaggga tgttgcaggt gctgtagagt tttggacaga taggttagga
      481 ttctcaagag actttgttga ggacgatttt gctggtgttg tcagggatga cgttacttta
      541 tttatctcag cagtccaaga tcaagttgtc cctgataata cattggcttg ggtctgggtc
      601 aggggtttag atgaattata tgctgaatgg tcagaagttg tatctacaaa cttcagagat
      661 gcttctggtc cagctatgac cgagattggt gaacagccat ggggtagaga atttgctttg
      721 agagatccag ctggaaattg tgttcatttt gttgctgaag aacaagatta aagtaactga
      781 caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt
      841 tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg
      901 cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct
      961 ggtcgctata ctggagttga gaccagacca ataaaaaacg cccggcggca accgagcgtt
     1021 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     1081 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1141 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1201 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1261 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1321 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1381 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1441 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1501 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     1561 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1621 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     1681 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1741 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     1801 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
