LOCUS       sequence-110241-        2174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK081
FEATURES             Location/Qualifiers
     source          1..2174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    14..517
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     terminator      complement(539..573)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(644..1303)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1218..1237
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1485..2073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1974..1993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agtatcacgt gctataaaaa taattataat ttaaattttt taatataaat
       61 atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc
      121 gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc
      181 tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaaaac cacacacgaa
      241 aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt
      301 ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg
      361 tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc
      421 aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa
      481 aagatacgag gcgcgtgtaa gttacaggca agcgatccgt cccgatgaga ccagaccaat
      541 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
      601 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
      661 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
      721 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
      781 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
      841 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
      901 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
      961 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     1021 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     1081 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     1141 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     1201 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     1261 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     1321 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     1381 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     1441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2161 gtggataacc gtag
//
