LOCUS       sequence-110242-        2667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes 2micron as a Type 7 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK082
FEATURES             Location/Qualifiers
     source          1..2667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..996
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      complement(1032..1066)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1137..1796)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1711..1730
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1978..2566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2467..2486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agtaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
       61 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
      121 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
      181 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
      241 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
      301 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
      361 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
      421 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
      481 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
      541 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
      601 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
      661 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
      721 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
      781 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
      841 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
      901 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcatcaga gcgcttttgg
      961 ttttcaaaag cgctctgaag ttcctatact ttctagctag agaataggaa cttcccgatg
     1021 agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     1081 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     1141 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     1201 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     1261 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     1321 tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca
     1381 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1441 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     1501 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1561 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1621 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1681 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1741 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1801 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1861 tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta
     1921 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1981 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2041 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2101 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2161 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2221 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2281 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2341 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2401 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2461 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2521 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2581 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2641 atcccctgat tctgtggata accgtag
//
