LOCUS       sequence-110251-        2848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SpecR-ColE1 as a Type 8a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK091
FEATURES             Location/Qualifiers
     source          1..2848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(22..813)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1243..1831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1732..1751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1952..1970
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1977..1995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2013..2024
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2031..2708
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      2726..2797
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2813..2840
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tcggtctcac cgagcggccg cttatttgcc gactaccttg gtgatctcgc ctttcacgta
       61 gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag
      121 ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc
      181 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg
      241 ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag
      301 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc
      361 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc
      421 cagatcaatg tcgatcgtgg ctggctcgaa gatacccgca agaatgtcat tgcgctgcca
      481 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac
      541 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc
      601 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac
      661 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg
      721 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg
      781 agtcgatact tcggcgatca ccgcttccct cataatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gtagcagcga
     1141 agtcgaggca tttctgtcct ggctggtcat gaccaaaatc ccttaacgtg agttttcgtt
     1201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1381 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1681 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1741 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1801 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1861 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1921 ggataaccgt gcggccgcca attgagacca gtccctatca gtgatagaga ttgacatccc
     1981 tatcagtgat agagatactg agcacggatc tgaaagagga gaaaggatct atggcgagta
     2041 gcgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
     2101 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt actcagaccg
     2161 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
     2221 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
     2281 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
     2341 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
     2401 tgcgtggtac taacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
     2461 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
     2521 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
     2581 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
     2641 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
     2701 gtgcttaata aggatctcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2761 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2821 ttcgggtggg cctttctgcg tttataag
//
