LOCUS       sequence-110253-        2186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes LEU2 5' Homology as a Type 8b part to be used in the Dueber 
            YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK093
FEATURES             Location/Qualifiers
     source          1..2186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(551..585)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(656..1315)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1230..1249
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1986..2005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcac aatacccaga agaacagtaa aataaagcaa ggtacgtgaa attaatattt
       61 ttaaatggtt ctaaccgatg ccgaagaact gcgcagtccg gttataacgt ctgacatgtc
      121 cttttttgat ttggaatcca accactcaag tgactctgtt catttacttt gcgaaaaata
      181 tacccacaaa ttgcccatcg aaagtgaatc gcaaaccacc ttcagactgg caccgacaaa
      241 gcaaagatta tacagacaga gtactttata cgtaccgtta agtctcaagc aaagggtttt
      301 cttatttact gaacgggtaa agagtatctg ggccggcttg ccaagatgca aaccgaataa
      361 gtatttcaaa gttgcatttg ccttagccgt cctgacacca ttggctattt ggatatttta
      421 tattgacttt cgtgtacatt gatcacatcg actgttctat tggcaaatga accacgggca
      481 ttgactattt ttcaggttac tactatatat tatcatcacg ggcaaggatt gtacccttga
      541 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
      601 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      661 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      721 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      781 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      841 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
      901 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      961 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     1021 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1081 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1141 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1201 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1261 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1321 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1381 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtag
//
