LOCUS       sequence-110263-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Vinculin-23
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..678)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      675..684
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taacagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
      121 cgcggccgct taacccagcc caggcttgcc cagtctgtgg ccctcgtggg ccagcagcca
      181 ctctttcacg gcgagcccgc cctcgtagcc ccccacgtcc aggtcgccct gcaccacccg
      241 gtggcagggg atcagaatgg gcacgggatt tccgctcagg gcggttttca cggcggcggt
      301 ggcggcggga ttgccggcca gggcggccag gtggctgtag ctgatgacct ctccgaactt
      361 caccactttc agcagtttcc acagcacctg gcgggtaaag ctctcctgct ggaacactgg
      421 gtggtgcagg gctggcacag ggaactcctc gatggcctca ggctggtgaa agtaggcgtt
      481 gagccaggcg gtggcctgca tcagtggctc tggtccgccc agcacggcgg ctggggcagg
      541 cacttccacg gcgtcggcgg cagatgttcc tttgcccagg aagatgatac ggtgcaggcc
      601 ctgttcgcac ccagacagtt ccagcttgcc cagagggcta tccagggtgg tgcgcttcat
      661 ttcgcagtct ttgtccatgg tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg
      721 cagaattccg ctgccaccgg actggtacca gggagtcttt ctaacccagc gcagtgtaaa
      781 tccagcatct gttcgaattt tgattgaagc
//
