LOCUS       sequence-110274-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS 
            controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..710
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     580..603
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      739..748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tggagctcac tcggaattca gtctactagt cagttcgagt ttatcattat caatactgcc
       61 atttcaaaga atacgtaaat aattaatagt agtgattttc ctaactttat ttagtcaaaa
      121 aattagcctt ttaattctgc tgtaacccgt acatgcccaa aatagggggc gggttacaca
      181 gaatatataa catcgtaggt gtctgggtga acagtttatt cctggcatcc actaaatata
      241 atggagcccg ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt
      301 gttttcttca ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag
      361 gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt
      421 aaatgatgac acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct
      481 attaccttct gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc
      541 tgaaattatt cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt
      601 ctgtaaatct atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt
      661 tttaaaacac caagaactta gtttcgaata aacacacata aacaaacaaa agatctgata
      721 tggatcgaat tagatctcgc caccatggac aagaagtatt ctatcggact ggccatcggg
//
