LOCUS       sequence-110281-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by 
            URA3 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1122
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(767..772,773..796,797..832,833..856)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 ttcgtacgct gcaggtcgac ggatcggtga cggtgctggt ttaattaaca tgtcgaaggg
       61 tgaagaactg ttcactggtg ttgtaccaat tttggttgaa ctggatggtg acgttaatgg
      121 tcataaattc tctgtaagtg gtgaaggtga aggtgacgct acttatggta aattgactct
      181 taagttcatc tgcacaactg gtaaattgcc agttccatgg ccaacattag taactacttt
      241 ttcttatggt gttcaatgtt ttgctagata ccctgatcat atgaagcaac atgacttctt
      301 taagtccgca atgcctgaag gatatgttca agaacgtaca attttcttta aggacgacgg
      361 aaactacaag acaagggctg aagtcaaatt tgagggagat accttagtaa acaggattga
      421 gcttaaggga attgacttta aagaggatgg aaacattcta ggccataaat tggagtacaa
      481 ttttaactct cacaacgtgt atattatggc tgataaacag aagaatggca tcaaagttaa
      541 ttttaaaata agacacaata tagaggatgg gagtgtgcag ctagcagatc actatcaaca
      601 aaatacccct ataggcgatg ggccggtctt gttgcccgat aatcattatc tatcaattca
      661 atcagcatta tctaaagatc ccaatgaaaa aagagatcac atggtgttat tagaatttgt
      721 cacggccgcg gggataaccc atggcatgga tgaattgtac aaaggtagtg cttggagtca
      781 tccacaattc gaaaaaggtg gaggttccgg aggtggatcg ggaggttctg cctggtctca
      841 tccacaattt gagaagtaag gtaccgcgcg cgccacttct aaataagcga atttcttatg
      901 atttatgatt tttattatta aataagttat aaaaa
//
