LOCUS       sequence-110283-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggagacca caatgggcgt aatcaagccc gacatgaaga tcaagctgaa gatggagggc
      121 aacgtgaatg gccacgcctt cgtgatcgag ggcgagggcg agggcaagcc ctacgacggc
      181 accaacacca tcaacctgga ggtgaaggag ggagcccccc tgcccttctc ctacgacatt
      241 ctgaccaccg cgttcgccta cggcaacagg gccttcacca agtaccccga cgacatcccc
      301 aactacttca agcagtcctt ccccgagggc tactcttggg agcgcaccat gaccttcgag
      361 gacaagggca tcgtgaaggt gaagtccgac atctccatgg aggaggactc cttcatctac
      421 gagatacacc tcaagggcga gaacttcccc cccaacggcc ccgtgatgca gaagaagacc
      481 accggctggg acgcctccac cgagaggatg tacgtgcgcg acggcgtgct gaagggcgac
      541 gtcaagcaca agctgctgct ggagggcggc ggccaccacc gcgttgactt caagaccatc
      601 tacagggcca agaaggcggt gaagctgccc gactatcact ttgtggacca ccgcatcgag
      661 atcctgaacc acgacaagga ctacaacaag gtgaccgttt acgagagcgc cgtggcccgc
      721 aactccaccg acggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct
      781 tcgatggcga ctgcgacccc cgtgccgccg cggatgggca gccgcgctgg cggcccca
//
