LOCUS       sequence-110289-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eVenus Strep-tag II followed by URA3 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1512
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             774..797
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatcggtg acggtgctgg tttaattaac atgtcgaagg
       61 gtgaagaact gttcactggt gttgtaccaa ttttggttga actggatggt gacgttaatg
      121 gtcataaatt ctctgtaagt ggtgaaggtg aaggtgacgc tacttatggt aaattgactc
      181 ttaagttgat ctgcacaact ggtaaattgc cagttccatg gccaacatta gtaactactc
      241 ttggttatgg tttgcaatgt tttgctagat accctgatca tatgaagcaa catgacttct
      301 ttaagtccgc aatgcctgaa ggatatgttc aagaacgtac aattttcttt aaggacgacg
      361 gaaactacaa gacaagggct gaagtcaaat ttgagggaga taccttagta aacaggattg
      421 agcttaaggg aattgacttt aaagaggatg gaaacattct aggccataaa ttggagtaca
      481 attacaactc tcacaacgtg tatattaccg ctgataaaca gaagaatggc atcaaagcca
      541 attttaaaat aagacacaat atagaggatg ggggtgtgca gctagcagat cactatcaac
      601 aaaatacccc tataggcgat gggccggtct tgttgcccga taatcattat ctatcatatc
      661 aatcagcatt atctaaagat cccaatgaaa aaagagatca catggtgtta ttagaatttg
      721 tcacggccgc ggggataacc catggcatgg atgaattgta caaaggtagt gcttggagtc
      781 atccacaatt cgaaaaataa ggtaccgcgc gcgccacttc taaataagcg aatttcttat
      841 gatttatgat ttttattatt aaataagtta taaaa
//
