LOCUS       sequence-110293-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by 
            URA3 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1422
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..763
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(767..772,773..796,797..832,833..856)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 ttcgtacgct gcaggtcgac ggatcggtga cggtgctggt ttaattaaca tgtctaaagg
       61 tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg
      121 tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acttacggta aattgacctt
      181 aaaatttatt tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt
      241 aggttatggt ttgatgtgtt ttgctagata cccagatcat atgaaacaac atgacttttt
      301 caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca aagatgacgg
      361 taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta atagaatcga
      421 attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat tggaatacaa
      481 ctataactct cacaatgttt acatcatggc tgacaaacaa aagaatggta tcaaggttaa
      541 cttcaaaatt agacacaaca ttgaagatgg ttccgttcaa ttagctgacc attatcaaca
      601 aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact tatcctatca
      661 atctgcctta tccaaagatc caaacgaaaa gagagaccac atggtcttgt tagaatttgt
      721 tactgctgct ggtattaccc atggtatgga tgaattgtac aaaggtagtg cttggagtca
      781 tccacaattc gaaaaaggtg gaggttccgg aggtggatcg ggaggttctg cctggtctca
      841 tccacaattt gagaagtaag gtaccgcgcg cgccacttct aaataagcga atttcttatg
      901 atttatgatt tttattatta aataagttat aaaaaaa
//
