LOCUS       sequence-110294-        9549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of GFP-Mps1 DK fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Foijer F, Xie SZ, Simon JE, Bakker PL, Conte N, Davis SH, Kregel E, 
            Jonkers J, Bradley A, Sorger PK
  TITLE     Chromosome instability induced by Mps1 and p53 mutation generates 
            aggressive lymphomas exhibiting aneuploidy-induced stress.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13427-32. doi: 
            10.1073/pnas.1400892111. Epub 2014 Sep 2.
  PUBMED    25197064
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400892111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-09-16- 16"; volume: "111"; issue: "37"; pages: 
            "13427-32"
COMMENT     Sequence Label: pRetroX GFP Mps1 DK
FEATURES             Location/Qualifiers
     source          1..9549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1603..1917
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1647..1665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1682..1700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1718..1736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1754..1772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1825..1843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1890..1914
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1931..1933,1934..1936,1937..2647)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1931..1933,1934..1936,1937..2647)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1976..1997)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2237..2256)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2584..2605
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4955..5454
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4990..5013)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5387..5406
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5475..6074
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5475..5494)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5971..5991
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6255..6680
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6783..6805)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6941..6961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6946..7275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7126..7261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7297..7316
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7397..7414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7568..8156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7648..7667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8327..9118,9119..9187))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8950..8969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9188..9292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9360..9378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9478..9549,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgaggccctt tcgtcttcac tcgagtttac tccctatcag
     1621 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta
     1681 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     1741 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg
     1801 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
     1861 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1921 agaaggatct atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1981 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2041 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2101 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     2161 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2221 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2281 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2341 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     2401 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2461 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2521 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2581 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2641 gtacaaggga ggacccggag gaggatccat ggaatccgag gatttaagtg gcagagaatt
     2701 gacaattgat tccataatga acaaagtgag agacattaaa aataagttta aaaatgaaga
     2761 ccttactgat gaactaagct tgaataaaat ttctgctgat actacaggta atgtcaaaaa
     2821 aagtaaacaa cttcttcaaa aagctgtaga acgtggagca gtaccactag aaatgctgga
     2881 aattgccctg cggaatttaa acctccaaaa aaagcagctg ctttcagagg aggaaaagaa
     2941 gaatttatca gcatctacgg tattaactgc ccaagaatca ttttccggtt cacttgggca
     3001 tttacagaat aggaacaaca gttgtgattc cagaggacag actactaaag ccaggttttt
     3061 atatggagag aacatgccac cacaagatgc agaaataggt taccggaatt cattgagaca
     3121 aactaacaaa actaaacagt catgcccatt tggaagagtc ccagttaacc ttctaaatag
     3181 cccagattgt gatgtgaaga cagatgattc agttgtacct tgttttatga aaagacaaac
     3241 ctctagatca gaatgccgag atttggttgt gcctggatct aaaccaagtg gaaatgattc
     3301 ctgtgaatta agaaatttaa agtctgttca aaatagtcat ttcaaggaac ctctggtgtc
     3361 agatgaaaag agttctgaac ttattattac tgattcaata accctgaaga ataaaacgga
     3421 atcaagtctt ctagctaaat tagaagaaac taaagagtat caagaaccag aggttccaga
     3481 gagtaaccag aaacagtggc aatctaagag aaagtcagag tgtattaacc agaatcctgc
     3541 tgcatcttca aatcactggc agattccgga gttagcccga aaagttaata cagagcagaa
     3601 acataccact tttgagcaac ctgtcttttc agtttcaaaa cagtcaccac caatatcaac
     3661 atctaaatgg tttgacccaa aatctatttg taagacacca agcagcaata ccttggatga
     3721 ttacatgagc tgttttagaa ctccagttgt aaagaatgac tttccacctg cttgtcagtt
     3781 gtcaacacct tatggccaac ctgcctgttt ccagcagcaa cagcatcaaa tacttgccac
     3841 tccacttcaa aatttacagg ttttagcatc ttcttcagca aatgaatgca tttcggttaa
     3901 aggaagaatt tattccatat taaagcagat aggaagtgga ggttcaagca aggtatttca
     3961 ggtgttaaat gaaaagaaac agatatatgc tataaaatat gtgaacttag aagaagcaga
     4021 taaccaaact cttgatagtt accggaacga aatagcttat ttgaataaac tacaacaaca
     4081 cagtgataag atcatccgac tttatgatta tgaaatcacg gaccagtaca tctacatggt
     4141 aatggagtgt ggaaatattg atcttaatag ttggcttaaa aagaaaaaat ccattgatcc
     4201 atgggaacgc aagagttact ggaaaaatat gttagaggca gttcacacaa tccatcaaca
     4261 tggcattgtt cacagtgatc ttaaaccagc taactttctg atagttgatg gaatgctaaa
     4321 gctaattgat tttgggattg caaaccaaat gcaaccagat acaacaagtg ttgttaaaga
     4381 ttctcaggtt ggcacagtta attatatgcc accagaagca atcaaagata tgtcttcctc
     4441 cagagagaat gggaaatcta agtcaaagat aagccccaaa agtgatgttt ggtccttagg
     4501 atgtattttg tactatatga cttacgggaa aacaccattt cagcagataa ttaatcagat
     4561 ttctaaatta catgccataa ttgatcctaa tcatgaaatt gaatttcccg atattccaga
     4621 gaaagatctt caagatgtgt taaagtgttg tttaaaaagg gacccaaaac agaggatatc
     4681 cattcctgag ctcctggctc atccatatgt tcaaattcaa actcatccag ttaaccaaat
     4741 ggccaaggga accactgaag aaatgaaata tgttctgggc caacttgttg gtctgaattc
     4801 tcctaactcc attttgaaag ctgctaaaac tttatatgaa cactatagtg gtggtgaaag
     4861 tcataattct tcatcctcca agacttttga aaaaaaaagg ggaaaaaaat gagcggccgc
     4921 gccggctcta gatcgcgaac gcgtgaattc taccgggtag gggaggcgct tttcccaagg
     4981 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     5041 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     5101 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     5161 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     5221 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     5281 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     5341 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     5401 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     5461 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     5521 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     5581 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     5641 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     5701 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     5761 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     5821 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     5881 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5941 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     6001 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     6061 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     6121 cccatgcatc ggcgtctcga gatatcagtg gtccaggctc tagttttgac tcaacaatat
     6181 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
     6241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     6301 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     6361 ggaacagatg gaacagggtc gaccctagag aaccatcaga tgtttccagg gtgccccaag
     6421 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     6481 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     6541 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     6601 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     6661 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     6721 caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa
     6781 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6841 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     6901 acccagtcac gtagcgatag cggagtgtag atccggctgt ggaatgtgtg tcagttaggg
     6961 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7021 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7081 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7141 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7201 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7261 ctaggctttt gcaaaaagct tactggctta actatgcggc atcagagcag attgtactga
     7321 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7381 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7441 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7501 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7981 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8641 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8881 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8941 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9001 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9061 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9301 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9361 ataggcgtat cacgaggccc tttcgtcttc aagaattagc ttggccattg catacgttgt
     9421 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
     9481 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9541 atatggagt
//
