LOCUS       sequence-110296-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eVenus Twin-Strep tag followed by URA3
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1522
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(93..290)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gcggccgcat aggccactag tggatctgat atcatcgatg aattcgagct cgttttcgac
       61 actggatggc ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga
      121 ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg
      181 cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa
      241 aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt acagattata
      301 attggccagt ctttttcaaa taagcattcc aaccagcatc tctatacctt ttaccttcaa
      361 tatctggatc tcttccttta ccaaacaatc ctctaccaac aatgataata tcagttccag
      421 tgctaacaac ttcatcaaca gttctatatt gttgtcctaa tccatcacct ttatcatcta
      481 atccaactcc aggtgtcata ataagccaat caaatccttc ttcttgtcca cccatatcac
      541 gttgggcaat aaatccaata acaaattcct tatcggattt agcaatttca acagtttttt
      601 gagaatattc tccatatgct aatgatccca ctgatgataa ttcagctaac atcaataacc
      661 ctcttggctc ttggttggtg gtggtttctt tagctccctg ttttaatcct tcaactactc
      721 cattcccagt gacaccatga gcattagtaa tatctgccca actactaatt ttataaactc
      781 caccaatata ttgtttcttc acggtattac caatatcagc aaattttcta tcttcaaaaa
      841 tcataaattg atgtttacgt gaaagttcta ataatggttc aatagtggat tcataggaaa
      901 aatcattgat tatatcaata tgagtcttga ttaagcatac ataaggaccc aatttatcaa
      961 ttaattcaag gaattcctta gtggtatcaa catcaattga tgcacataaa ttggttttct
     1021 tcagttccat taatcgaaat aatcgttgtg ctactgg
//
