LOCUS       sequence-110297-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eVenus Twin-Strep tag followed by URA3
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1522
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(770..775,776..799,800..835,836..859)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 agcttcgtac gctgcaggtc gacggatcgg tgacggtgct ggtttaatta acatgtcgaa
       61 gggtgaagaa ctgttcactg gtgttgtacc aattttggtt gaactggatg gtgacgttaa
      121 tggtcataaa ttctctgtaa gtggtgaagg tgaaggtgac gctacttatg gtaaattgac
      181 tcttaagttg atctgcacaa ctggtaaatt gccagttcca tggccaacat tagtaactac
      241 tcttggttat ggtttgcaat gttttgctag ataccctgat catatgaagc aacatgactt
      301 ctttaagtcc gcaatgcctg aaggatatgt tcaagaacgt acaattttct ttaaggacga
      361 cggaaactac aagacaaggg ctgaagtcaa atttgaggga gataccttag taaacaggat
      421 tgagcttaag ggaattgact ttaaagagga tggaaacatt ctaggccata aattggagta
      481 caattacaac tctcacaacg tgtatattac cgctgataaa cagaagaatg gcatcaaagc
      541 caattttaaa ataagacaca atatagagga tgggggtgtg cagctagcag atcactatca
      601 acaaaatacc cctataggcg atgggccggt cttgttgccc gataatcatt atctatcata
      661 tcaatcagca ttatctaaag atcccaatga aaaaagagat cacatggtgt tattagaatt
      721 tgtcacggcc gcggggataa cccatggcat ggatgaattg tacaaaggta gtgcttggag
      781 tcatccacaa ttcgaaaaag gtggaggttc cggaggtgga tcgggaggtt ctgcctggtc
      841 tcatccacaa tttgagaagt aaggtaccgc gcgcgccact tctaaataag cgaatttctt
      901 atgatttatg atttt
//
