LOCUS       sequence-110300-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by 
            URA3 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1112
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             774..797
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatcggtg acggtgctgg tttaattaac atgtcgaagg
       61 gtgaagaact gttcactggt gttgtaccaa ttttggttga actggatggt gacgttaatg
      121 gtcataaatt ctctgtaagt ggtgaaggtg aaggtgacgc tacttatggt aaattgactc
      181 ttaagttcat ctgcacaact ggtaaattgc cagttccatg gccaacatta gtaactactt
      241 tttcttatgg tgttcaatgt tttgctagat accctgatca tatgaagcaa catgacttct
      301 ttaagtccgc aatgcctgaa ggatatgttc aagaacgtac aattttcttt aaggacgacg
      361 gaaactacaa gacaagggct gaagtcaaat ttgagggaga taccttagta aacaggattg
      421 agcttaaggg aattgacttt aaagaggatg gaaacattct aggccataaa ttggagtaca
      481 attttaactc tcacaacgtg tatattatgg ctgataaaca gaagaatggc atcaaagtta
      541 attttaaaat aagacacaat atagaggatg ggagtgtgca gctagcagat cactatcaac
      601 aaaatacccc tataggcgat gggccggtct tgttgcccga taatcattat ctatcaattc
      661 aatcagcatt atctaaagat cccaatgaaa aaagagatca catggtgtta ttagaatttg
      721 tcacggccgc ggggataacc catggcatgg atgaattgta caaaggtagt gcttggagtc
      781 atccacaatt cgaaaaataa ggtaccgcgc gcgccacttc taaataagcg aatttcttat
      841 gatttatgat ttttattatt aaataagtta taaaaaa
//
