LOCUS       sequence-110305-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating LexA::QF drivers from MiMIC lines with inserts into coding 
            introns in Phase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..117
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             286..339
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             346..366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actcactata gggcgaattg gagctcgcta gcgatgtagg tcacggtctc gaagccgcgg
       61 tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg
      121 tccatcatga tggtcgactc tagaagtcga tccaacatgg cgacttgtcc catccccggc
      181 atgtttaaat atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc
      241 gcaggtggcg gaggttccgg tggaagcgga ggtagcggcg gatccgaggg ccgcggcagc
      301 ctgctgacct gcggcgatgt ggaggagaac cccgggccca tgccacccaa gaagaagcga
      361 aaagtagaag atccaatgaa ggctctcacg gcccgacaac aggaagtttt tgatttgata
      421 cgggatcata tatcccaaac gggtatgcct ccgacccgcg cagagatagc acagcgactg
      481 ggctttcgat cgcctaacgc cgcggaggag cacttgaagg cactggcccg caagggtgtc
      541 attgaaatcg tgtccggtgc gagccgcgga atccggctgt tgcaggagga ggaagagggc
      601 ctgccactgg tgggacgcgt ggccgctggc gagccgctgc tggcccagca acacatagag
      661 ggacactatc aggtggaccc ctccttgttt aagccaaatg ctgatttcct gttgcgggtg
      721 tcgggaatgt ccatgaagga catcggtatt atggatggtg acctcctcgc cgtccataag
      781 acacaggatg tcaggaacgg ccaggtagtc gttgccagga tagacgatga ggtcactgtg
      841 aaa
//
