LOCUS       sequence-110310-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a 
            LEU2 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Lin TY, Voronovsky A, Raabe M, Urlaub H, Sander B, Golas MM
  TITLE     Dual tagging as an approach to isolate endogenous chromatin 
            remodeling complexes from Saccharomyces cerevisiae.
  JOURNAL   Biochim Biophys Acta. 2015 Mar;1854(3):198-208. doi: 
            10.1016/j.bbapap.2014.11.009. Epub 2014 Dec 5.
  PUBMED    25486077
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbapap.2014.11.009"; journalName: "Biochim Biophys Acta";
            date: "2015-03"; volume: "1854"; issue: "3"; pages: "198-208"
COMMENT     Sequence Label: pGS1034
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..115
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             131..196
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             224..244
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             284..457
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             458..631
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     terminator      687..874
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(742..766)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttggttctgg ttctggtaaa agaagatgga agaagaattt
       61 tattgctgtt tctgctgcta atagatttaa gaaaatttct tcttctggtg ctttgtctgc
      121 ttctggttct gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa
      181 ggatgacgat gacaaggatt atgatattcc aactactgct agcgagaatt tgtattttca
      241 gggtgagctc aaaaccgcgg ctcttgcgca acacgatgaa gccgtggaca acaaattcaa
      301 caaagaacaa caaaacgcgt tctatgagat cttacattta cctaacttaa acgaagaaca
      361 acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta accttttagc
      421 agaagctaaa aagctaaatg atgctcaggc gccgaaagta gacaacaaat tcaacaaaga
      481 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
      541 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
      601 taaaaagcta aatggtgctc aggcgccgaa agtagacgcg aattccgcgg ggaagtcaac
      661 ctgagtcgac cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
      721 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
      781 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
      841 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa
      901 tatagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
      961 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     1021 ccgtacattt agcccataca tccc
//
