LOCUS       sequence-110315-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating Gal4 drivers from MiMIC lines with inserts into coding 
            introns in Phase 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(0)-T2A-Gal4
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..118
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             285..338
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             339..779
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 gactcactat agggcgaatt ggagctcgct agcgatgtag gtcacggtct cgaagccgcg
       61 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg
      121 gtccatcatg atggtcgact ctagaagtcg atccaacatg gcgacttgtc ccatccccgg
      181 catgtttaaa tatactaatt attcttgaac taattttaat caaccgattt atctctcttc
      241 cgcaggtggg aggttccggt ggaagcggag gtagcggcgg atccgagggc cgcggcagcc
      301 tgctgacctg cggcgatgtg gaggagaacc ccgggcccat gaagctgctg agcagcatcg
      361 agcaggcctg cgatatctgc cgcctgaaga agctgaagtg cagcaaggag aagcccaagt
      421 gcgccaagtg cctgaagaac aactgggagt gccgctacag ccccaagacc aagcgcagcc
      481 ccctgacccg cgcccacctg accgaggtgg agagccgcct ggagcgcctg gagcagctgt
      541 tcctgctgat cttcccccgc gaggatctgg atatgatcct gaagatggat agcctgcagg
      601 atatcaaggc cctgctgacc ggcctgttcg tgcaggataa cgtgaacaag gatgccgtga
      661 ccgatcgcct ggccagcgtg gagaccgata tgcccctgac cctgcgccag caccgcatca
      721 gcgccaccag cagcagcgag gagagcagca acaagggcca gcgccagctg accgtgagca
      781 tcgatagcgc cgcccaccac gataacagca ccatccccct ggatttcatg ccccgcgatg
      841 ccctgcacgg cttcgattgg agcgaggagg atgatatgag cgatggcctg cccttcctga
      901 agaccgatcc caacaacaac ggcttcttcg gcgatggcag cctgctgtgc atcctgcgca
      961 gcatcggctt caagcccgag aactacacca acagcaacgt gaaccgcct
//
