LOCUS       sequence-110324-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H6-TRX-Smt3-GB1-NpuDnaEN102-CBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Guerrero F, Ciragan A, Iwai H
  TITLE     Tandem SUMO fusion vectors for improving soluble protein expression 
            and purification.
  JOURNAL   Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019.
  PUBMED    26297996
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019."
COMMENT     Sequence Label: pCARSF63
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..384
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     CDS             391..681
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
ORIGIN
        1 tgtttaactt taataaggag atataccatg ggcagcagcc atcatcatca tcatcacggc
       61 agcgataaaa ttattcacct gactgacgac agttttgaca cggatgtact caaagcggac
      121 ggggcgatcc tcgtcgattt ctgggcagag tggtgcggtc cgtgcaaaat gatcgccccg
      181 attctggatg aaatcgctga cgaatatcag ggcaaactga ccgttgcaaa actgaacatc
      241 gatcaaaacc ctggcactgc gccgaaatat ggcatccgtg gtatcccgac tctgctgctg
      301 ttcaaaaacg gtgaagtggc ggcaaccaaa gtgggtgcac tgtctaaagg tcagttgaaa
      361 gagttcctcg acgctaacct ggcgggaact agtgactcag aagtcaatca agaagctaag
      421 ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga
      481 tcttcagaaa tcttctttaa gatcaaaaag accactcctt taagaaggct gatggaagcg
      541 ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga
      601 attcaagctg atcagacccc tgaagatttg gacatggagg ataacgatat tattgagact
      661 cacagagaac agattggtgg atcccatatg cagtacaaac ttatcctgaa cggtaaaacc
      721 ctgaaaggtg aaaccaccac cgaagctgtt gacgctgcta ccgcggaaaa agttttcaaa
      781 cagtacgcta acgacaacgg tgttgacggt gaatggacct acgacgacgc taccaaaacc
      841 tacaccgtaa cggaaggatc ctgtttaagc tatgaaacgg aaatattgac agtagaatat
      901 ggattattac cgattggtaa aattgtagaa aagcgcatcg aatgtactgt ttatagcgtt
      961 gataataatg gaaatattta tacacaacct gtagcacaat ggcacga
//
