LOCUS       sequence-110333-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of St1 dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             170..190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      121 ccagcctacc ggttaaggtg aacgcgtgca ggctggcgcc accatggccc ctaaaaagaa
      181 acgcaaggtc gaattcggaa gcgacctggt gctgggcctg gccatcggca tcggcagcgt
      241 gggcgtgggc atcctgaaca aggtgaccgg cgagatcatc cacaagaaca gtcgcatctt
      301 ccctgctgct caggctgaga acaacctggt gcgccgcacc aaccgccagg gtcgccggct
      361 tgctcgccgc aagaagcacc ggcgcgtgcg cctgaaccgc ctgttcgagg agagcggcct
      421 gatcaccgac ttcaccaaga tcagcatcaa cctgaacccc taccagctgc gcgtgaaggg
      481 cctgaccgac gagctgagca acgaggagct gttcatcgcc ctgaagaaca tggtgaagca
      541 ccgcggcatc agctacctgg acgacgccag cgacgacggc aacagcagcg tgggcgacta
      601 cgcccagatc gtgaaggaga acagcaagca gctggagacc aagacccccg gccagatcca
      661 gctggagcgc taccagacct acggccagct gcgcggcgac ttcaccgtgg agaaggacgg
      721 caagaagcac cgcctgatca acgtgttccc caccagcgcc taccgcagcg aggccctgcg
      781 catcctgc
//
