LOCUS       sequence-110335-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-SSFV-dCas9-GFP
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             57..77
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgactgagtc gcccaccggt taaggtgaac gcgtgcaggc tggcgccacc atggccccta
       61 aaaagaaacg caaggtcgaa ttcggaagcg gaagcgacaa gaagtactcc attgggctcg
      121 ctatcggtac caacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca
      181 aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggag
      241 ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc
      301 gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga
      361 tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg
      421 ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg
      481 aaaagtaccc aaccatatat catctgagga agaagctggt agacagtact gataaggctg
      541 acttgcggtt gatctatctc gcgctggcgc acatgatcaa atttcgggga cacttcctca
      601 tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc
      661 agacttacaa tcagcttttc gaggagaacc cgatcaacgc atccggcgtt gacgccaaag
      721 caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc
      781 ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc
      841 ccaactttaa atctaacttc gacctggccg aagatgccaa gctgcaactg
//
