LOCUS       sequence-110337-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-dCas9-3XGFP
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     45..69
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             174..194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac
       61 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      121 cgatccagcc taccggttaa ggtgaacgcg tgcaggctgg cgccaccatg gcccctaaaa
      181 agaaacgcaa ggtcgaattc ggaagcggaa gcgacaagaa gtactccatt gggctcgcta
      241 tcggtaccaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa
      301 aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc attggagccc
      361 tcctgttcga ctccggggag acggccgaag ccacgcggct caaaagaaca gcacggcgca
      421 gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg
      481 ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata
      541 aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa
      601 agtacccaac catatatcat ctgaggaaga agctggtaga cagtactgat aaggctgact
      661 tgcggttgat ctatctcgcg ctggcgcaca tgatcaaatt tcggggacac ttcctcatcg
      721 agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa ctggttcaga
      781 cttacaatca gctttt
//
