LOCUS       sequence-110339-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Nm dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nmdCas9-3XGFP
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     39..63
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             168..188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctaccgg ttaaggtgaa cgcgtgcagg ctggcgccac catggcccct aaaaagaaac
      181 gcaaggtcga attcggaagc gccgccttca agcccaaccc catcaactac atcctgggcc
      241 tggccatcgg catcgccagc gtgggctggg ccatggtgga gatcgacgag gacgagaacc
      301 ccatctgcct gatcgacctg ggtgtgcgcg tgttcgagcg cgctgaggtg cccaagactg
      361 gtgacagtct ggctatggct cgccggcttg ctcgctctgt tcggcgcctt actcgccggc
      421 gcgctcaccg ccttctgcgc gctcgccgcc tgctgaagcg cgagggtgtg ctgcaggctg
      481 ccgacttcga cgagaacggc ctgatcaaga gcctgcccaa cactccttgg cagctgcgcg
      541 ctgccgctct ggaccgcaag ctgactcctc tggagtggag cgccgtgctg ctgcacctga
      601 tcaagcaccg cggctacctg agccagcgca agaacgaggg cgagaccgcc
//
