LOCUS       sequence-110347-       11266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for peroxisomes mCherry-SKL expressed under 
            AtUBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 11266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMU-PERr
FEATURES             Location/Qualifiers
     source          1..11266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3835..3859
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3862..4569
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4275..4294
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4583..4607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4837..4857)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4865..4882)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4865..4881)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4874..4896)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4970..4994
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6294..6923
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7357..8424
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8490..8684
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8920..8942)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9028..9168
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9083..9102
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9183..9200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9354..9942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9434..9453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10186..10977)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgag tctagctcaa
     1861 cagagctttt aacccaaatt ggtacaatag aatacaactt tagatcataa ttctcaaaag
     1921 aaagagattc cttagctatt ctatctgcca ctccatttcc ttctcggctt gtatgcacaa
     1981 gcataaaatc ctcaaacttg ctaagtagat actttatgtc ttggataatt ggattgagac
     2041 ttgacaagca taactttcat gtaaccaaag acacaagttg ctgagaatcc acctcaaaaa
     2101 tgatcttcct ataattgaat cgggataatg acagcacagc ccatctaaga gcctccactt
     2161 ctacttccag cacgcttctt acttttacca cagctcttgc acctaaccat aacaccttcc
     2221 ctgtatgatc gcgaagcacc caccctaagc cacattttaa tccttctgtt ggccatgccc
     2281 catcaaagtt gcacttaacc caagattgtg gtggagcttc ccatgtttct cgtctgtccc
     2341 gacggtgttg tggttggtgc tttccttaca ttctgagcct ctttccttct aatccactca
     2401 tctgcatctt cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc
     2461 accagctcgt ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca
     2521 ttcttcagat caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact
     2581 gatgatctag gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg
     2641 tgtatcattc ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca
     2701 cgagtgttaa atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca
     2761 agaataaatc gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga
     2821 tacaaaagtc attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa
     2881 aaattaaaag aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa
     2941 gaaattcact gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag
     3001 gaaaagaaat aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga
     3061 cccacggttc aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata
     3121 atgctaaaaa aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga
     3181 ccgttagaaa ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc
     3241 ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat
     3301 aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta
     3361 ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct
     3421 tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa
     3481 tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct
     3541 gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat
     3601 gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt
     3661 agatatcatc ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat
     3721 ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt
     3781 ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggcaagt
     3841 ttgtacaaaa aagcaggctt catggtgagc aagggcgagg aggataacat ggccatcatc
     3901 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     3961 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4021 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4081 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4141 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4201 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4261 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4321 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4381 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4441 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4501 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4561 ctgtacaagt ctaaactgta agacccagct ttcttgtaca aagtggaata agcggccatg
     4621 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     4681 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     4741 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     4801 taaaatttct aattcctaaa ccaaaatcca gtgacgcaac tttattatac atagttgata
     4861 attcactggc cgtcgtttta caacgtcgtg actgggaaaa cgagctcaag cttagcttga
     4921 gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat
     4981 attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc
     5041 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     5101 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     5161 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     5221 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     5281 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     5341 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     5401 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     5461 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     5521 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     5581 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     5641 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     5701 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     5761 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     5821 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     5881 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     5941 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     6001 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     6061 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     6121 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     6181 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     6241 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     6301 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     6361 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     6421 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     6481 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     6541 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     6601 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     6661 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     6721 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     6781 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     6841 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     6901 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     6961 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     7021 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     7081 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     7141 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     7201 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     7261 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     7321 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
     7381 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     7441 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     7501 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     7561 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     7621 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     7681 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     7741 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     7801 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     7861 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     7921 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     7981 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     8041 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     8101 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     8161 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     8221 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     8281 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     8341 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     8401 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     8461 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
     8521 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     8581 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     8641 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     8701 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     8761 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     8821 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     8881 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     8941 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     9001 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     9061 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9121 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     9181 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9241 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9301 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9361 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9421 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9481 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9541 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9601 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9661 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9721 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9781 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9841 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9901 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9961 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    10021 gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac
    10081 ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaatc gcttgagtta
    10141 acgccggcga agcggcgtcg gcttgaacga atttctagct agacattatt tgccgactac
    10201 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
    10261 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
    10321 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
    10381 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
    10441 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
    10501 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
    10561 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
    10621 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
    10681 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
    10741 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
    10801 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
    10861 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
    10921 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt cccccatgat
    10981 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
    11041 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
    11101 cccaaaaaaa catgtcataa caagaagcca tgaaaaccgc cactgcgccg ttaccaccgc
    11161 tgcgttcggt caaggttctg gaccagttgc gtgacggcag ttacgctact tgcattacag
    11221 cttacgaacc gaacgaggct tatgtccact gggttcgtgc ccgaat
//
