LOCUS       sequence-110359-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expression of St1 sgRNA1.1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pLH-stsgRNA1.1
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             355..660
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     539..558
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaggacgaaa caccggtgtc ttcgaggctt actaaaagcc agataacagt atgcgtattt
       61 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
      121 aagaggtgtg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
      181 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
      241 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
      301 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag
      361 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
      421 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
      481 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
      541 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
      601 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
      661 accctgcagg acgaagacca gtctttgtac tctcagaaat gcagaagcta caaagataag
      721 gcttcatgcc gaaatcaaca ccctgtcatt ctatggcagg gtgttttttt gaattctcga
      781 cctcgagaca aat
//
