LOCUS       sequence-110363-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expression of St1 sgRNA2.1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pLH-stsgRNA2.1
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             354..659
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     538..557
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aggacgaaac accggtgtct tcgaggctta ctaaaagcca gataacagta tgcgtatttg
       61 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
      121 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
      181 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
      241 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
      301 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt
      361 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
      421 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
      481 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
      541 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
      601 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
      661 ccctgcagga cgaagaccag tctttgtact ctcaagagaa atcttgcaga agctacaaag
      721 ataaggcttc atgccgaaat caacaccctg tcattctatg gcagggtgtt tttttgaatt
      781 ctcgacctcg agacaaatgg c
//
