LOCUS       sequence-110366-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry 
            expressed under MtBCP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-TGNVHAr
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..714)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(282..301)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     551..569
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    716..740
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgcttacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta
       61 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt
      121 gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc
      181 gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc
      241 ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg
      301 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
      361 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
      421 ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt
      481 cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag
      541 ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
      601 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
      661 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca ccatttccac
      721 tttgtacaag aaagctgggt cgattaaagc gaaagagaaa ggcttgaact tgtaaccatc
      781 tccattgaag aacttcccca tgaattctac c
//
