LOCUS       sequence-110377-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating Gal4 drivers from MiMIC lines with inserts into coding 
            introns in Phase 0..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..119
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             286..339
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             340..780
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcgc tagcgatgta ggtcacggtc tcgaagccgc
       61 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct
      121 ggtccatcat gatggtcgac tctagaagtc gatccaacat ggcgacttgt cccatccccg
      181 gcatgtttaa atatactaat tattcttgaa ctaattttaa tcaaccgatt tatctctctt
      241 ccgcaggtgg gaggttccgg tggaagcgga ggtagcggcg gatccgaggg ccgcggcagc
      301 ctgctgacct gcggcgatgt ggaggagaac cccgggccca tgaagctgct gagcagcatc
      361 gagcaggcct gcgatatctg ccgcctgaag aagctgaagt gcagcaagga gaagcccaag
      421 tgcgccaagt gcctgaagaa caactgggag tgccgctaca gccccaagac caagcgcagc
      481 cccctgaccc gcgcccacct gaccgaggtg gagagccgcc tggagcgcct ggagcagctg
      541 ttcctgctga tcttcccccg cgaggatctg gatatgatcc tgaagatgga tagcctgcag
      601 gatatcaagg ccctgctgac cggcctgttc gtgcaggata acgtgaacaa ggatgccgtg
      661 accgatcgcc tggccagcgt ggagaccgat atgcccctga ccctgcgcca gcaccgcatc
      721 agcgccacca gcagcagcga ggagagcagc aacaagggcc agcgccagct gaccgtgagc
      781 atcgatagcg ccgcccacca cgataacagc accatccccc tggatttcat gccccgcgat
      841 gccctgcacg gctt
//
