LOCUS       sequence-110378-        4511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AND gate reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pAND_Reporter
FEATURES             Location/Qualifiers
     source          1..4511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..792
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRTDQLEEFVHYVKGEITKVVGK"
     promoter        944..978
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(1091..1804)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGNVNGHKFSVSGEGEGDAAYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1131..1151)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     1746..1774
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             1811..1822
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(1853..1902)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      1984..2055
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2071..2098
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      2235..2329
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2821..3043
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3091..4041
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
       61 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
      121 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
      181 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
      241 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
      301 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
      361 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
      421 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
      481 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
      541 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
      601 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
      661 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
      721 tcgcgcacag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
      781 gtcggcaaat aatactagct ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt
      841 ttctttaaaa ccgaaaagat tacttcgcgt ttgccacctg acgtctaaga attttcgaaa
      901 aatggcctcc caaatcgggg ggcctttttt attgataaca aaattgacag ctagctcagt
      961 cctaggtata atgctagcag ttcgatgaga gcgataacgg acggcgcaga aggggagtag
     1021 ctcttcgccg gaccgtcgac atactgctca gctcgtctta agcgtagttt tcgtcgtttg
     1081 ctgcaggcct tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact
     1141 caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg
     1201 acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat
     1261 aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt
     1321 tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt
     1381 ccaacttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc
     1441 tattaacaag tgtatcacct tcaaacttga cttcagcacg tgtcttatag ttcccgtcat
     1501 ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt
     1561 catgccgttt catatgatct gggtatcttg aaaagcattg aacaccataa gtcaaagtag
     1621 tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa
     1681 gttttccgta tgctgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa
     1741 cattaccatc taattcaaca agaattggga caactccagt gaaaagttct tctcctttac
     1801 tcatctagta tttctcctct ttctctagat taaacaaaat tatttgtaga ggctgtttcg
     1861 tcctcacgga ctcatcagac cggaaagcac atccggtgac agctagccaa gagcagtgcc
     1921 ttgcagaagt tctcgggcga gaatctcgcg tagacacata catgagctac tagagccagg
     1981 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2041 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2101 tatagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2161 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaactgtc agaccaagtt
     2221 tacgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
     2281 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcactag
     2341 ggacagtaag acgggtaagc ctgttgatga taccgctgcc ttactgggtg cattagccag
     2401 tctgaatgac ctgtcacggg ataatccgaa gtggtcagac tggaaaatca gagggcagga
     2461 actgctgaac agcaaaaagt cagatagcac cacatagcag acccgccata aaacgccctg
     2521 agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga atccataaaa
     2581 ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca gcgaactgaa
     2641 tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg aatattcagc
     2701 gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg tctgttttgt
     2761 agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga ctaaagtagt
     2821 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
     2881 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
     2941 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
     3001 acaactcaaa ggaaaaggac atgtaattat cattgactag cccatctcaa ttggtatagt
     3061 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
     3121 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
     3181 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
     3241 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
     3301 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
     3361 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
     3421 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
     3481 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
     3541 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
     3601 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
     3661 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
     3721 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
     3781 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     3841 acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc tttaactgca
     3901 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagcatgat
     3961 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
     4021 gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg aagcatcaag
     4081 actaacaaac aaaagtagaa caactgttca ccgttagata tcaaagggaa aactgtccat
     4141 atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac gagtttttgg
     4201 tgcatttaaa gctgttcacc atgaacagat cgacaatgta acgattatca aaaaggatct
     4261 tcacctagat ccttttaaat taaaaatgaa gcttcgttgt gtctcaaaat ctctgatgtt
     4321 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     4381 gtaatacaag gggtgtttac tagaggttga tcgggcacgt aagaggttcc aactttcacc
     4441 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     4501 aggaagctaa a
//
