LOCUS       sequence-110381-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for ZAP-70 tyrosine kinase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Cadra S, Gucciardi A, Valignat MP, Theodoly O, Vacaflores A, Houtman
            JC, Lellouch AC
  TITLE     ROZA-XL, an improved FRET based biosensor with an increased dynamic 
            range for visualizing Zeta Associated Protein 70 kD (ZAP-70) 
            tyrosine kinase activity in live T cells.
  JOURNAL   Biochem Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5.
            doi: 10.1016/j.bbrc.2015.02.117.
  PUBMED    25735979
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5. doi: 
            10.1016/j.bbrc.2015.02.117."
COMMENT     Sequence Label: ROZA-XL
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     484..503
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatagaaga gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgat ttctagaaag cttagatctt tacttataca gctcgttcat gccctcggtg
      121 atgccggcgg cggtcaggaa ctccagcagc accatgtggt cccgcttctc gttggggtcc
      181 ttgaacaggg cgctctggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      241 ccgatggggg tgttctgctg gtagtggtcg gccagctgca cgccgccgtc ctcgatgttg
      301 tgccggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt gatgtacacg
      361 ttgtggctgt tgtagttgta ctccagcttg tggcccagga tgttgccgtc ctccttgaag
      421 tcgatgccct tcagctcgat ccggttcacc agggtgtcgc cctcgaactt cacctcggcc
      481 cgggtcttgt agttgccgtc gtccttgaag aagatggtcc gctcctgcac gtagccctcg
      541 ggcatggcgc tcttgaagaa gtcgtgctgc ttcatgtggt cggggtaccg ggcgaagcac
      601 tgcacgccgt agcccagggt ggtcaccagg gtgggccagg gcacgggcag cttgccggtg
      661 gtgcacagca gcttcagggt cagcttgccg taggtggcgt cgccctcgcc ctcgccgctc
      721 acgctgaact tgtggccgtt cacgtcgccg tccagctcca ccaggatggg caccacgccg
      781 gtgaacagct cctcgccctt gctcaccatg agctccagtt cctgggacac attcacatac
      841 tcccggctgc ctcccccgtt attacctgca gacccgccat tgttatttcc cccgttattg
      901 ccttctatgt cccgtaagaa tatctgctgg ttcctggaca cggatgttga tctgtggtaa
      961 tctaccagct cattcaaaga attaaacttc accaccc
//
