LOCUS       sequence-110384-         428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter 
            vector to select iChon cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N
  TITLE     Direct induction of chondrogenic cells from human dermal fibroblast 
            culture by defined factors.
  JOURNAL   PLoS One. 2013 Oct 16;8(10):e77365. doi: 
            10.1371/journal.pone.0077365. eCollection 2013.
  PUBMED    24146984
REFERENCE   2  (bases 1 to 428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-16"; volume: "8(10):e77365. doi"; pages: " 
            10.1371/journal.pone.0077365. eCollection 2013"
COMMENT     Sequence Label: pLe6-Delta -hLP-mcs/(EGFP-IresPuro-hInt)
FEATURES             Location/Qualifiers
     source          1..428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     197..218
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      260..269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(307..331)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
       61 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      121 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      181 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      241 agctcctcgc ccttgctcac catggtggcg accggtggat ccagtcgact gaattggttc
      301 ctttaaagcc tgcttttttg tacaaacttg ggtcgaattg acactcgagc tcccggcact
      361 gctccctcct cggtggctgc cgcttctgtg tgtccccggc caccctggcg cccagagccc
      421 ccacctcg
//
