LOCUS       sequence-110386-         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating dVP16AD hemidrivers from MiMIC lines with inserts into 
            coding introns in Phase 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..119
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             286..339
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             370..390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcgc tagcgatgta ggtcacggtc tcgaagccgc
       61 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct
      121 ggtccatcat gatggtcgac tctagaagtc gatccaacat ggcgacttgt cccatccccg
      181 gcatgtttaa atatactaat tattcttgaa ctaattttaa tcaaccgatt tatctctctt
      241 ccgcaggtgg gaggttccgg tggaagcgga ggtagcggcg gatccgaggg ccgcggcagc
      301 ctgctgacct gcggcgatgt ggaggagaac cccgggccca tggataaagc ggaattaatt
      361 cccgagcctc caaaaaagaa gagaaaggtc gaattgggta ccgccccccc caccgacgtg
      421 tccctgggcg atgagctgca cctggatggc gaggatgtgg ccatggccca cgccgatgcc
      481 ctggatgact tcgacctgga tatgctgggc gatggcgaca gccccggccc cggcttcacc
      541 ccccacgata gcgcccccta cggcgccctg gacatggccg atttcgagtt cgagcaaatg
      601 ttcaccgacg ccctgggcat cgatgagtac ggcggcgcta gcgg
//
