LOCUS       sequence-110393-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F 
            mutated control sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Cadra S, Gucciardi A, Valignat MP, Theodoly O, Vacaflores A, Houtman
            JC, Lellouch AC
  TITLE     ROZA-XL, an improved FRET based biosensor with an increased dynamic 
            range for visualizing Zeta Associated Protein 70 kD (ZAP-70) 
            tyrosine kinase activity in live T cells.
  JOURNAL   Biochem Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5.
            doi: 10.1016/j.bbrc.2015.02.117.
  PUBMED    25735979
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5. doi: 
            10.1016/j.bbrc.2015.02.117."
COMMENT     Sequence Label: ROZA-XL YF
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     486..505
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agctatagaa tagggccctc tagatgcatg ctcgagcggc cgccagtgtg atggatatct
       61 gcagaattcg atttctagaa agcttagatc tttacttata cagctcgttc atgccctcgg
      121 tgatgccggc ggcggtcagg aactccagca gcaccatgtg gtcccgcttc tcgttggggt
      181 ccttgaacag ggcgctctgg tagctcaggt agtggttgtc gggcagcagc acggggccgt
      241 cgccgatggg ggtgttctgc tggtagtggt cggccagctg cacgccgccg tcctcgatgt
      301 tgtgccggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatgtaca
      361 cgttgtggct gttgtagttg tactccagct tgtggcccag gatgttgccg tcctccttga
      421 agtcgatgcc cttcagctcg atccggttca ccagggtgtc gccctcgaac ttcacctcgg
      481 cccgggtctt gtagttgccg tcgtccttga agaagatggt ccgctcctgc acgtagccct
      541 cgggcatggc gctcttgaag aagtcgtgct gcttcatgtg gtcggggtac cgggcgaagc
      601 actgcacgcc gtagcccagg gtggtcacca gggtgggcca gggcacgggc agcttgccgg
      661 tggtgcacag cagcttcagg gtcagcttgc cgtaggtggc gtcgccctcg ccctcgccgc
      721 tcacgctgaa cttgtggccg ttcacgtcgc cgtccagctc caccaggatg ggcaccacgc
      781 cggtgaacag ctcctcgccc ttgctcacca tgagctccag ttcctgggac acattcacaa
      841 actcccggct gcctcccccg ttattacctg cagacccgcc attgttattt cccccgttat
      901 tgccttctat gtcccgtaag aatatctgct ggttcctgga cacggatgtt gatctgtggt
      961 aatctaccag ctcattcaaa
//
