LOCUS       sequence-110397-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating QF2 drivers from MiMIC lines with inserts into coding 
            introns in Phase 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..116
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             284..337
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 actactatag ggcgaattgg agctcgctag cgatgtaggt cacggtctcg aagccgcggt
       61 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
      121 ccatcatgat ggtcgactct agaagtcgat ccaacatggc gacttgtccc atccccggca
      181 tgtttaaata tactaattat tcttgaacta attttaatca accgatttat ctctcttccg
      241 caggtcggga ggttccggtg gaagcggagg tagcggcgga tccgagggcc gcggcagcct
      301 gctgacctgc ggcgatgtgg aggagaaccc cgggcccatg ccacccaagc gcaaaacgct
      361 taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa acgccgacgg
      421 acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc tcactaacat
      481 cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag cacgctcaaa
      541 gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga aatgcgacgg
      601 aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt atcaggcttc
      661 gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg ccctcgcctg
      721 gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg tccgtgacgc
      781 cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc gactccatgc
      841 ccgttgggct actagccgtg tcaataagag cattacccgc ctcctccgtc agttggagct
      901 ccctcctacc gccacggcta cggcctcgat
//
