LOCUS       sequence-110399-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""Reporter of Zap-70 Activity"" Fluorescent reporter of ZAP-70 
            tyrosine kinase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Randriamampita C, Mouchacca P, Malissen B, Marguet D, Trautmann A, 
            Lellouch AC
  TITLE     A novel ZAP-70 dependent FRET based biosensor reveals kinase 
            activity at both the immunological synapse and the antisynapse.
  JOURNAL   PLoS One. 2008 Jan 30;3(1):e1521. doi: 10.1371/journal.pone.0001521.
  PUBMED    18231606
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-01-30"; volume: "3(1):e1521. doi"; pages: " 
            10.1371/journal.pone.0001521"
COMMENT     Sequence Label: ROZA
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2..19
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(join(114..827,828..830,831..833))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(159..180)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     508..527
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     767..788
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 catttaggtg acactataga atagggccct ctagatgcat gctcgagcgg ccgccagtgt
       61 gatggatatc tgcagaattc gattggctga ttatgatctg aaagcttaga tctttacttg
      121 tacagctcgt ccatgccgtg agtgatcccg gcggcggtca cgaactccag caggaccatg
      181 tgatcgcgct tctcgttggg gtctttgctc agcttggact ggtagctcag gtagtggttg
      241 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      301 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
      361 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
      421 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      481 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      541 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      601 tggtcggggt agcgggcgaa gcacttcagg ccgtagccga aggtggtcac gagggtgggc
      661 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      721 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      781 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgagctcc
      841 tcactctcag gaacattcac gtaatcttca caagacccgc cattgttatt tcccccgtta
      901 ttacctgcag acccgccatt gttatttccc ccgttattgc cttctatgtc ccgtaagaat
      961 atctgctggt tcctggacac ggatgttgat ctgtggtaat ctaccagctc
//
