LOCUS       sequence-110400-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To study the role of the differential rates of G-beta-gamma 
            translocation in modulating signaling dynamics in a cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Giri L, Patel AK, Karunarathne WK, Kalyanaraman V, Venkatesh KV, 
            Gautam N
  TITLE     A G-protein subunit translocation embedded network motif underlies 
            GPCR regulation of calcium oscillations.
  JOURNAL   Biophys J. 2014 Jul 1;107(1):242-54. doi: 10.1016/j.bpj.2014.05.020.
  PUBMED    24988358
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.05"; journalName: "Biophys J"; date: "2014-07-1-
            1"; volume: "107"; issue: "1"; pages: "242-54"
COMMENT     Sequence Label: pcDNA3.1 mCherry Gamma 3
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..725
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(163..181)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     431..450
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtaccgagct cggatccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
       61 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      121 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      181 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      241 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      301 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      361 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      421 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      481 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
      541 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      601 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      661 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      721 acaagggtac catgaaaggg gagacccccg ttaacagcac tatgagtatt ggtcaagcac
      781 gcaagatggt ggaacagctt aagattgagg ccagcttgtg ccggataaag gtgtccaagg
      841 cagcagcaga cctgatgaca tactgtgatg cccacgcttg tgaggacccc ctcatcactc
      901 ctgtgcccac ttccgagaac cccttccggg agaagaagtt cttctgtgcg ctcctctgag
      961 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccn
     1021 cgnc
//
