LOCUS       sequence-110402-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent Reporter for ZAP-70 tyrosine kinase activity-control YF 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Randriamampita C, Mouchacca P, Malissen B, Marguet D, Trautmann A, 
            Lellouch AC
  TITLE     A novel ZAP-70 dependent FRET based biosensor reveals kinase 
            activity at both the immunological synapse and the antisynapse.
  JOURNAL   PLoS One. 2008 Jan 30;3(1):e1521. doi: 10.1371/journal.pone.0001521.
  PUBMED    18231606
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-01-30"; volume: "3(1):e1521. doi"; pages: " 
            10.1371/journal.pone.0001521"
COMMENT     Sequence Label: ROZA YF
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(144..146,147..149,150..662)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(189..210)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(450..469)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgcggccgc caccatgggc tgtgtctgca
      121 gctcaaaccc tgaagatgac aacatggtga gcaagggcga ggagctgttc accggggtgg
      181 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caggttcagc gtgtccggcg
      241 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      301 agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca
      361 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      421 acgtccagga gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      481 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      541 aggacggcaa catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata
      601 tcaccgccga caagcagaag aacggcatca aggcccactt caagatccgc cacaacatcg
      661 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      721 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
      781 acgagaagcg cgatcacatg gtcctgaagg agttcgtgac cgccgcccgc atgcataaac
      841 cacatccgtg gttttttggc aaaatcccca gagccaaggc agaagaaatg ctcagcaaac
      901 agcggcatga cggggccttc ctgatccgag agagcgagag cgctcctggg gacttctccc
      961 tgtccgtcaa gtttggaaat gatgtgcagc acttcaaggt gctccgcgac ggagccggga
     1021 agtatttcct gtgggtggtg aagtttaatt ctttgaatga gctggtagat taccacagat
     1081 caac
//
