LOCUS       sequence-110421-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HA tagged dominant active MEK5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Buschbeck M, Eickhoff J, Sommer MN, Ullrich A
  TITLE     Phosphotyrosine-specific phosphatase PTP-SL regulates the ERK5 
            signaling pathway.
  JOURNAL   J Biol Chem. 2002 Aug 16;277(33):29503-9. Epub 2002 May 31.
  PUBMED    12042304
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-08-16"; volume: "277(33)"; pages: "29503-9. Epub
            2002 May 31"
COMMENT     Sequence Label: pcDNA3-MEK5DD-HA
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..38
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(61..87)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctgatcgc gagctctagc atttaggtga cactatagcc gcggccgcta gctcgagtta
       61 agcgtaatct ggaacatcgt atgggtaggg ccctctagac gggggcccct gctggctccg
      121 cctctcctcc agcgcccggc acacccacat ggacaccacg gcggcatttc catcattgaa
      181 ctgcacgatg aacgggtggc ccatcaattc ttcaggtgct ggcctttctt ttggctgttt
      241 tcgcatacac tgagtgatga aatgtacaaa tggctccgag aactctccaa ttggaaggac
      301 gggcgaatcc tcatcaacaa tgcactgcag aagctggaga ggcattaaag atccctggtt
      361 tttctgaatc tgaggatatg gaaacctccc aagagcaagc tccataaaag agattcctaa
      421 gctccagaca tcagaatgaa ttccatactg ctcccctgaa atcctttcag gcgccatata
      481 agcatttgtt ccaacata
//
