LOCUS       sequence-110427-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make 
            Gal4 driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..39
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    155..224
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             392..445
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             446..886
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 tcgagataac ttcgtataat gtatgctata cgaagttatc aattggctta ttgggatttc
       61 aatttaagat gctgctaaat aaagttagtc acttacggac tggggaggca ccccgtcatc
      121 actcgccaga aatgcaattg atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg
      181 gcgtgccctt gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatgg
      241 tcgactctag aagtcgatcc aacatggcga cttgtcccat ccccggcatg tttaaatata
      301 ctaattattc ttgaactaat tttaatcaac cgatttatct ctcttccgca ggtcgggagg
      361 ttccggtgga agcggaggta gcggcggatc cgagggccgc ggcagcctgc tgacctgcgg
      421 cgatgtggag gagaaccccg ggcccatgaa gctgctgagc agcatcgagc aggcctgcga
      481 tatctgccgc ctgaagaagc tgaagtgcag caaggagaag cccaagtgcg ccaagtgcct
      541 gaagaacaac tgggagtgcc gctacagccc caagaccaag cgcagccccc tgacccgcgc
      601 ccacctgacc gaggtggaga gccgcctgga gcgcctggag cagctgttcc tgctgatctt
      661 cccccgcgag gatctggata tgatcctgaa gatggatagc ctgcaggata tcaaggccct
      721 gctgaccggc ctgttcgtgc aggataacgt gaacaaggat gccgtgaccg atcgcctggc
      781 cagcgtggag accgatatgc ccctgaccct gcgccagcac cgcatcagcg ccaccagcag
      841 cagcgaggag agcagcaaca agggccagcg ccagctgacc gtgagcatcg atagcgccgc
      901 ccaccacgat aacagcacca tccccctgga tttcatgccc cgcgatg
//
