LOCUS       sequence-110430-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make 
            Gal4 driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     169..187
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    complement(219..252)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    368..437
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cagcgctgac tttgagtgga atgtcatttt gagtgagagg taatcgaaag aacctggtac
       61 atcaaatacc cttggatcga agtaaattta aaactgatca gataagttca atgatatcca
      121 gtgcagtaaa aaaaaaaaat gtttttttta tctactttcc gcaaaaatgg gttttattaa
      181 cttacataca tactagaatt aggccttcta gtggatctat aacttcgtat agcatacatt
      241 atacgaagtt attcaaattt aaataataac aattataatt tttactctgg cataattgta
      301 tgcaatttgc aaatgcagct tcgtttttta ggggtgtgaa tatttattgt atgatgtagg
      361 tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
      421 actccacctc acccatctgg tccatcatga tggtcgacaa gcttagaagt tcaaatgggc
      481 tttcatattt acttttttct ctgagtgggt aaggtcattg tggggtctta agggctaaag
      541 ggtagtgatg atgaaggcat ttgatgccat tgttcaataa cttacctgcc ggaacctccg
      601 cttccaccgc tacctccgga tccaaacgag tttttaagca aactcactcc ctgacaataa
      661 aaacgaatta tctttaaata aatatattct atttattaac caagtagcta atatagctaa
      721 aatcaatttg ttgctaactt aaaactaaca taattatgta ttagttattt acattctttt
      781 ggttggttac aaccaaataa ataatatcac ttttaaaaac ttaagccaga actgaataat
      841 ttctatttgg ctt
//
