LOCUS       sequence-110433-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic
            loci using the Crispr/Cas technology. Can be converted by cassette 
            exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pT-GEM(0)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..138
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             284..337
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             338..778
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 actactatgg gcgacccacc ggtgcatgcg cggccgcccc agggtcagaa gcggttttcg
       61 ggagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg tagggtcgcc
      121 gacatgacac aaggggtttc tagaagtcga tccaacatgg cgacttgtcc catccccggc
      181 atgtttaaat atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc
      241 gcaggtggga ggttccggtg gaagcggagg tagcggcgga tccgagggcc gcggcagcct
      301 gctgacctgc ggcgatgtgg aggagaaccc cgggcccatg aagctgctga gcagcatcga
      361 gcaggcctgc gatatctgcc gcctgaagaa gctgaagtgc agcaaggaga agcccaagtg
      421 cgccaagtgc ctgaagaaca actgggagtg ccgctacagc cccaagacca agcgcagccc
      481 cctgacccgc gcccacctga ccgaggtgga gagccgcctg gagcgcctgg agcagctgtt
      541 cctgctgatc ttcccccgcg aggatctgga tatgatcctg aagatggata gcctgcagga
      601 tatcaaggcc ctgctgaccg gcctgttcgt gcaggataac gtgaacaagg atgccgtgac
      661 cgatcgcctg gccagcgtgg agaccgatat gcccctgacc ctgcgccagc accgcatcag
      721 cgccaccagc agcagcgagg agagcagcaa caagggccag cgccagctga ccgtgagcat
      781 cgatagcgcc gcccaccacg ataacagcac catccccctg gatttcatgc cccgcgatgc
      841 cctgcacggc tt
//
