LOCUS       sequence-110434-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cerulean-H2B-6
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(28..405)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(494..697)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(523..543)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcaccatg gtggcgaccg gtggatcctt agcgctggtg tacttggtga tggccttagt
       61 accctcggac acggcgtgct tggccaactc cccaggcagc agcaggcgca cggccgtctg
      121 gatctccctg gaggtgatgg tcgagcgctt gttgtaatgc gccaggcggg aagcctcacc
      181 tgcgatgcgc tcgaaaatgt cgttcacaaa cgaattcatg atgcccatgg ccttggacga
      241 aatgccggtg tcagggtgga cctgcttcag aaccttgtac acatagatgg aatagctctc
      301 cttgcggctg cgcttgcgct tcttgccgcc tttcttctgc gccttagtca ccgccttctt
      361 ggagcccttt ttcggggcgg gagcagactt cgctggctct ggcatggtgg cggtaccgtc
      421 gactgcagaa ttcgaagctt gagctcgaga tctgagtccg gtagcgctag cggatctgac
      481 ggttcactaa accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
      541 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
      601 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
      661 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
      721 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactggggc ataatgccag
      781 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
//
