LOCUS       sequence-110435-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic
            loci using the Crispr/Cas technology. Can be converted by cassette 
            exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pT-GEM(0)
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..145
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     protein_bind    152..185
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    192..326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(216..235)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     270..289
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(598..618)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          748..814
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 cattaccctc ctaagggacc tccccactag tggtaccggc gcgcccccag gtcagaagcg
       61 gttttcggga gtagtgcccc aactggggta acctttgagt tctctcagtt gggggcgtag
      121 ggtcgccgac atgacacaag gggttctcga gataacttcg tataatgtat gctatacgaa
      181 gttatgctag cgatccagac atgataagat acattgatga gtttggacaa accacaacta
      241 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      301 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      361 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      421 ctgattatga tctctagtca aggcactata catcaaatat ttttccataa ttttcttgta
      481 tagcagtgca gctttttcct ttgtggtgta aatagcaaag caagcaagag ttctattact
      541 aaacacagca tgactcaaaa aacttagcaa ttctgaagga aagtccttgg ggtcttctac
      601 ctttctcttc ttttttggag gagtagaatg ttgagagtca gcagtagcct catcatcact
      661 agatggcatt tcttctgagc aaaacaggtt ttcctcatta aaggcattcc accactgctc
      721 ccattcatca gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt
      781 aacacattat acacttaaaa aattttatat ttaccttaga gctttaaatc tctgtaggta
      841 gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt cncaaagatc ctaaaggaac
      901 agatggtggc gtccctcggt tctttccata c
//
