LOCUS       sequence-110440-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic
            loci using the Crispr/Cas technology. Can be converted by cassette 
            exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pT-GEM(1)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..128
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             276..329
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             330..770
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 ggcgacccac cggtgcatgc gcggccgccc caggtcagaa gcggttttcg ggagtagtgc
       61 cccaactggg gtaacctttg agttctctca gttgggggcg tagggtcgcc gacatgacac
      121 aaggggtttc tagaagtcga tccaacatgg cgacttgtcc catccccggc atgtttaaat
      181 atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc gcaggtggcg
      241 gaggttccgg tggaagcgga ggtagcggcg gatccgaggg ccgcggcagc ctgctgacct
      301 gcggcgatgt ggaggagaac cccgggccca tgaagctgct gagcagcatc gagcaggcct
      361 gcgatatctg ccgcctgaag aagctgaagt gcagcaagga gaagcccaag tgcgccaagt
      421 gcctgaagaa caactgggag tgccgctaca gccccaagac caagcgcagc cccctgaccc
      481 gcgcccacct gaccgaggtg gagagccgcc tggagcgcct ggagcagctg ttcctgctga
      541 tcttcccccg cgaggatctg gatatgatcc tgaagatgga tagcctgcag gatatcaagg
      601 ccctgctgac cggcctgttc gtgcaggata acgtgaacaa ggatgccgtg accgatcgcc
      661 tggccagcgt ggagaccgat atgcccctga ccctgcgcca gcaccgcatc agcgccacca
      721 gcagcagcga ggagagcagc aacaagggcc agcgccagct gaccgtgagc atcgatagcg
      781 ccgcccacca cgataacagc accatccccc tggatttcat gccccgcgat gccctgca
//
